Overview and typical use
HiQ Sil C4-5 uses a C4 stationary phase. Lower hydrophobicity for fast elution and less retentive reversed-phase methods. The phase should be selected after considering analyte polarity, ionization, aromaticity and the selectivity needed to resolve critical pairs. Method suitability must be confirmed experimentally.
Operating guidance
Use HPLC-grade solvents, filter samples where appropriate and avoid abrupt changes between immiscible mobile phases. Introduce flow gradually, monitor pressure and allow complete equilibration before evaluating retention. Remove non-volatile buffers before storage. Avoid exceeding the limits stated in the manufacturer’s documentation.
Typical applications
Potential uses include pharmaceutical assay and impurity methods, chemical purity testing, food-related analysis, environmental research, natural-product profiling and academic method development, provided the analytes and mobile phase are compatible with the phase.
Frequently asked questions
What is HiQ Sil C4-5?
HiQ Sil C4-5 is a C4 silica-based HPLC column family with 5 µm particles and a listed pore size of 120 Å.
What is its main separation mode?
Its primary use follows the chemistry of the bonded phase. Reversed-phase products use aqueous-organic mobile phases, while polar and bare-silica phases may be used in normal-phase or other validated modes.
Which dimensions are available?
The catalogue lists multiple 4.0 mm and 4.6 mm internal-diameter analytical columns in lengths from 35 mm to 300 mm, depending on the part number.
Are guard columns available?
Yes. Matching cartridge guard columns are listed, and a compatible guard-column adaptor is required.
Are prices shown online?
No. Contact Akira Analytical Solutions for a quotation and availability.
How should the column be conditioned?
Condition gradually with a fully compatible mobile phase until pressure and detector baseline are stable.
How should it be stored?
Flush out buffers and salts, use a compatible storage solvent and cap both ends to prevent drying or contamination.
How can I obtain technical support?
Send the analyte, mobile phase, instrument, current column and desired separation details to sales@akiralabs.com.
